RLU and studies using the luciferase reporter gene
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چکیده
منابع مشابه
Accelerating the pace of luciferase reporter gene assays.
Luciferase reporter gene assays have gained more importance because of their easy readout, high sensitivity and lack of environmental waste disposal problems. However, several obstacles remain that have prohibited a wider use and the implementation of this type of assay in high-throughput screening programs: (i) Measurements need to be carried out within an active enzyme reaction, and the asses...
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The introduction of exogenous DNA sequences into cultured cells is a cornerstone in the study of mammalian gene expression. For most cell types, the efficiency of stable gene transfer is usually quite low, which mandates the use of selectable markers to isolate a population of transfected cells with the desired phenotype. In simple gene expression experiments, only one type of selectable marker...
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To study the coordination of different lipid synthesis pathways during membrane biogenesis it is useful to work with an experimental system where membrane biogenesis occurs rapidly and in an inducible manner. We have found that phagocytosis of latex beads is practical for these purposes as cells rapidly synthesize membrane lipids to replenish membrane pools lost as wrapping material during part...
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The effects of cyclic AMP (cAMP)-elevating agents on the activity of cis-acting gene promoter sequences are frequently studied using the luciferase-reporter-gene system. The aim of the present study was to assess whether cAMP-elevating agents induce any changes in the level of luciferase activity independently of a transcriptional activation of promoter elements. Chloramphenicol acteyltransfera...
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Transfection of desired genetic materials into cells is an inevitable procedure in biomedical research studies. While numerous methods have been described, certain types of cells are resistant to many of these methods and yield low transfection efficiency(1), potentially hindering research in those cell types. In this protocol, we present an optimized transfection procedure to introduce lucifer...
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ژورنال
عنوان ژورنال: Nature Biotechnology
سال: 1998
ISSN: 1087-0156
DOI: 10.1038/nbt0898-702b